Abstract: Background: Adipose-derived stem cells secrete various cytokines that promote hair growth. Objective: To describe our experience of hair regeneration therapy using adipose-derived stem cell conditioned medium. Results: We performed the hair regeneration therapy in numerous Japanese patients and reported good results. We described characteristics of the commercialized conditioned medium, treatment methods, and future directions. Conclusion: Treatment using adipose-derived stem cell-conditioned medium is highly effective and may represent a new therapy for alopecia.